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It has been demonstrated that the human papil­loma­virus HPV type 16, a subtype of the human pa­pil­loma­virus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive. HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate human papillomavirus-related oropharyngeal cancer protein and the retinoblastoma-type pro­tein RBP involved in the regulation of proliferation and cell death.

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More than half of the patients recognized being chronic alcohol and tobacco users. Conclusions: The genotyping method was validated according to kit instructions. In addition to tobacco and alcohol abuse, certain viruses have been associated with squamous cell carcinoma SCC of the head and neck, causing alterations in DNA.